The pCnu plasmid was isolated from the Sm-delicate colonies, and mutations were identified by DNA sequencing of the cnu gene (Solgent, Taejon, Korea)
The EcoRI-EcoRV restriction fragment of pHL204 was changed with the EcoRIEcoRV restriction fragment of pHL562, pHL1104, or pHL1104* finishing the assembly of the substrate plasmids, pOri14, pHL1105, and pHL1105*. To…