July 2016

The membrane was then washed, blocked and incubated with Streptavidin-Alkaline Phosphatase conjugate and created with CDP Star reagent in accordance to manufacturer’s recommendations (Ambion Brightstar Biodetect package) and was exposed to X-ray movie for two h or overnight

Even with this, the truth that miR-709 represses the transcription of nascent Egr2 transcripts (Fig. 5B) indicates that there is a system to recruit miRNAs to accessible promoter sequences. In…