WT Salmonella induced significantly more IL-6 secretion as measured in infected mouse serum than did the WTAvrA+

May 18, 2017

n the presence of serum albumin . Cy3 NHS Ester biological activity However, the overall activity curves for the two surfactant preparations were very similar, with both reaching minimum surface tensions of,1 mN/m by 10 min of bubble pulsation. The ability of synthetic DEPN-8+1.5% Mini-B to exhibit comparable surface tension lowering to CLSE in the presence of 3 mg/ml albumin is a positive finding, since prior work has established that CLSE is more resistant to this plasma protein than several other current clinical exogenous surfactants. tensions of 461 mN/m and,1 mN/m . CLSE had equivalent minimum surface tension values of 762 mN/m and,1 mN/m at these times of 18522853 bubble pulsation. When surfactant concentration was raised to 2.5 mg/ml, dynamic surface activity was increased for all surfactants. At 2.5 mg/ml, DEPN- Surface-active behavior of DEPN-8+1.5% or 3% by weight Mini-B on the captive bubble surfactometer The interfacial behavior of DEPN-8+1.5% or 3% Mini-B is shown during 10 successive cycles of compression/expansion on the captive bubble surfactometer in 4 Synthetic Lung Surfactant synthetic mixtures were equivalent to CLSE in reaching minimum surface tensions of,1 mN/m on all ten recorded cycles of captive bubble compression/expansion at either a quasi-static rate or at a dynamic rate. However, maximum surface tension values for DEPN-8+1.5% or 3.0% Mini-B for all cycles were greater than those of CLSE at the quasi-static and dynamic compression rates studied on the captive bubble. DISCUSSION The results of this study show that a binary synthetic lung surfactant containing DEPN-8+1.5% by weight Mini-B peptide had substantial surface activity that in several aspects approached the clinically-relevant bovine surfactant extract CLSE. Moreover, DEPN-8+1.5% Mini-B was fully resistant to chemical degradation when incubated in vitro with PLA2, while CLSE was severely degraded by this enzyme. Mini-B and DEPN-8 had direct intermolecular interactions 10481938 based on plasmon resonance binding affinity and on deconvolution analyses of FTIR spectra indicating a modified peptide secondary structure in multilayers with DEPN-8. The adsorption of DEPN-8+1.5% Mini-B was greatly increased compared to DEPN8 alone, although adsorption of the binary synthetic surfactant was less than that of CLSE. DEPN-8+1.5% Mini-B had overall dynamic surface tension lowering ability in pulsating bubble studies that was similar to CLSE at a low surfactant phospholipid concentration of 0.5 mg/ml, with both surfactants reaching minimum surface tensions of,1 mN/m after 10 min of cycling. DEPN-8+1.5% Mini-B and CLSE also had comparable activity in reaching minimum surface tensions,1 mN/m in the presence of serum albumin at a surfactant concentration of 2.5 mg/ml on the pulsating bubble. Complementary Synthetic Lung Surfactant captive bubble studies showed that DEPN-8+1.5% or 3% Mini-B and CLSE all reached minimum surface tensions,1 mN/m when compressed under either quasi-static or dynamic conditions. However, maximum surface tension values for DEPN-8+1.5% Mini-B were higher than for CLSE on both the pulsating and captive bubble surfactometers. The sum of these findings show that DEPN-8 and Mini-B form an interactive and highly surface-active binary mixture, and support the continued development of related fully-synthetic exogenous lung surfactants containing novel lipids and SP-B peptides. We have previously reported the high surface activity and inhibition resistance of model surfactants containing DEPN-8 or a C