Rimentally derived oleoyl-ACP thioesterase AAD28187.1 in E. guineensis [151], with 97 and 89

May 29, 2018

Rimentally derived oleoyl-ACP thioesterase AAD28187.1 in E. guineensis [151], with 97 and 89 BLASTP identity respectively, and toChan et al. Biology Direct (2017) 12:Page 15 ofNP_001292940.1 from J. curcas (69 identity, 76 positives) and XP_007049712.1 from T. cacao (72 identity, 90 positives). Both these sequences have high homology and formed a clade with other characterized plant FATA genes. The remaining four could not be differentiated via sequence analysis but expression data suggested that they function as FATB to hydrolyze saturated acyl-ACPs. EgFATB_1 is not expressed in the mesocarp but has very high expression in the kernel, indicating that it is mainly involved in fatty acid chain termination in the kernel (Fig. 9f). As oil palm accumulates 48 C12:0 and 15 C14:0 in its kernel oil, EgFATB_1 probably encodes for a thioesterase with substrate SCR7 custom synthesis specificity for medium chains, i.e. lauryl- or myristoyl-ACP thioesterase. EgFATB_2 and EgFATB_3, only moderately expressed in the mesocarp and kernel, are probably involved in the formation of C16:0 since the acid accumulates to 44 in the mesocarp oil and 15 in the kernel oil. The remaining acyl-ACP thioesterase (EgFATB_4) was only detected at very low levels in both the mesocarp and kernel, and may code for stearoyl-ACP thioesterase as palm oil and palm kernel oil only contain 3.7?.1 [135] and 0.5? [152] stearic acid, respectively. Comparison of the genomic locations of the FA biosynthesis genes in the oil palm genome showed that three genes, namely EgFABF, EgFABH and EgFAD3, showed duplication events (Additional file 3: Figure S17). This is in accordance with the segmental duplications of chromosome arms reported by Singh et al. [5]. The study identified and characterized 42 key genes involved in FA biosynthesis in E. guineensis. This is the first study to identify key FA biosynthesis genes in both the oil palm mesocarp and kernel PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25679764 through sequence and gene expression analysis. The comprehensive information will help pave the way to an understanding of the different mechanisms involved in producing the unique fatty acid profiles of palm mesocarp and kernel oils.following GOslim process categories: responses to abiotic stimulus, responses to endogenous stimulus, RNA translation, and responses to stress. We found approximately oneseventh of the oil palm genes identified to be intronless. Two hundred ten R genes grouped in six classes based on their protein domain structures were also identified. Lipid-, especially FA-related genes, are of interest in oil palm where, in addition to their roles in specifying oil yield and quality, also contribute to the plant organization and are important for biotic and abiotic stress signaling. We identified 42 key genes involved in oil palm FA biosynthesis, which will be especially useful for oil palm breeders. The results from our study will facilitate understanding of the plant genome organization, and be an important resource for further comparative and evolutionary analysis. The study of oil palm genes will facilitate future advances in the regulation of gene function in the crop, and provide a theoretical foundation for marker-assisted breeding for increased oil yield and elevated oleic and other valuable fatty acids.Reviewers’ commentsReviewer’ report 1: Alexander Kel, Genexplain, Germany Reviewer commentsConclusions An integrated gene prediction pipeline was developed, enabling annotation of the African oil palm genome, and deriving a.