Otides by a novel depurination controlled process. Nucleic Acids Res. 2010;38:2522?0. Kabadi AM, Ousterout DG,

April 20, 2021

Otides by a novel depurination controlled process. Nucleic Acids Res. 2010;38:2522?0. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Multiplex Flufenoxuron Autophagy CRISPR/Cas9based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014;42, e147. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells. Mol Cell. 2014;54:698?ten. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector program. Sci Rep. 2014;4:5400. Ranganathan V, Wahlin K, Maruotti J, Zack DJ. Expansion in the CRISPR-Cas9 genome targeting space by way of the use of H1 promoter-expressed guide RNAs. Nat Commun. 2014;five:4516. Xu XM, Yoo MH, Carlson BA, Gladyshev VN, Hatfield DL. Simultaneous knockdown on the expression of two genes applying a number of shRNAs and subsequent knock-in of their expression. Nat Protoc. 2009;4:1338?eight.Aparicio-Prat et al. BMC Genomics (2015) 16:Web page 15 of31. Li W, Hardwick MJ, Rosenthal D, Culty M, Papadopoulos V. Peripheral-type benzodiazepine receptor overexpression and knockdown in human breast cancer cells indicate its prominent role in tumor cell proliferation. Biochem Pharmacol. 2007;73:491?03. 32. Roelz R, Pilz IH, Mutschler M, Pahl HL. Of mice and guys: human RNA polymerase III promoter U6 is much more Effective than its murine homologue for shRNA expression from a lentiviral vector in each human and murine progenitor cells. Exp Hematol. 2010;38:792?. 33. Huang J, Zhou N, Watabe K, Lu Z, Wu F, Xu M, et al. Extended non-coding RNA UCA1 promotes breast tumor development by suppression of p27 (Kip1). Cell Death Dis. 2014;5, e1008. 34. Harrow J, Frankish A, Gonzalez JM, Tapanari E, Diekhans M, Kokocinski F, et al. GENCODE: the reference human genome annotation for The ENCODE Project. Genome Res. 2012;22:1760?four. 35. Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, et al. Landscape of transcription in human cells. Nature. 2012;489:101?. 36. Landry JJ, Pyl PT, Rausch T, Zichner T, Tekkedil MM, Stutz AM, et al. The genomic and transcriptomic landscape of a HeLa cell line. G3 (Bethesda). 2013;three:1213?4. 37. Adey A, Burton JN, Kitzman JO, Hiatt JB, Lewis AP, Martin BK, et al. The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line. Nature. 2013;500:207?1. 38. Li J, Shou J, Guo Y, Tang Y, Wu Y, Jia Z, et al. Effective inversions and duplications of mammalian regulatory DNA components and gene clusters by CRISPR/Cas9. J Mol Cell Biol. 2015;7:284?8. 39. Kraft K, Geuer S, Will AJ, Chan WL, Paliou C, Borschiwer M, et al. Deletions, inversions, duplications: engineering of structural variants making use of CRISPR/Cas in mice. Cell Rep. 2015 doi:10.1016/j.celrep.2015.01.016 40. Gutschner T, Hammerle M, Eissmann M, Hsu J, Kim Y, Hung G, et al. The noncoding RNA MALAT1 is often a essential regulator with the metastasis phenotype of lung cancer cells. Cancer Res. 2013;73:1180?. 41. Wang T, Wei JJ, Sabatini DM, Lander ES. Genetic screens in human cells working with the CRISPR-Cas9 technique. Science. 2014;343:80?. 42. Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K. Genome-wide recessive genetic screening in mammalian cells having a lentiviral CRISPR-guide RNA library. Nat Biotechnol. 2014;32:267?three. 43. Svensen N, Diaz-Mochon JJ, Bradley M. Microarray generation of thousand-member oligonucleotide libraries. PLoS One particular. 2011;6, e24906. 44. Chari R, Mali P, Moosburner M, Chu.