Rabbit anti-N-Cadherin Polyclonal Antibody

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Product Details FAQ Citations(3) Video Pictures Documents |Overview |Description Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density |Synonym Cadherin-2; CDw325; Neural cadherin; CDH2; CDHN; NCAD |Host Rabbit |Specificity The antibody detects endogenous level of total N-Cadherin protein. |Species Reactivity Human, Mouse, Rat |Applications WB 1:500-1:1000, IHC 1:50-200, IF 1:200 , ELISA(peptide) 1:20000-1:40000 |Immunogen A synthesized peptide derived from Human CDH2(Accession P19022), corresponding to amino acid residues V739-L759. |Properties |Form Liquid |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Monoclonal Antibody |Clone 329-12 |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background The cell adhesion molecule (CAM), N-cadherin, has emerged as an important oncology therapeutic target. N-cadherin is a transmembrane glycoprotein mediating the formation and structural integrity of blood vessels. Its expression has also been documented in numerous types of poorly differentiated tumours [PMID: 25533096]. |Post-translational modifications Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).May be phosphorylated by OBSCN. |Cellular localization Endoplasmic reticulum;Extracellular region or secreted;Plasma Membrane; |UniProt P19022 | Western blot analysis of extract cefrom HT29 lls using N-Cadherin antibody |Tips:This product is for research use only. Not for use in diagnostic prodcedures.